GSM3192001: Unfixed_SetC; Homo sapiens; RNA-Seq - SRA

SRX4221219: GSM3192001: Unfixed_SetC; Homo sapiens; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 21.1M spots, 1.6G bases, 622.5Mb downloads

Submitted by: NCBI (GEO)

Study: Comparison of single-cell transcriptomics quality between unfixed cells and cells that were fixed and mock stained according to the RAID procedure

PRJNA476320 • SRP150624 • All experiments • All runs

show Abstracthide Abstract

Cell fixation, permeabilization and antibody staining of could have adverse effects on the quality of single cell transcriptomics data. To assess the effects of the RAID procedure, which includes such steps, we performed a direct comparison of single cell transcriptomics by CELseq2 using unfixed and RAID-processed cells. Quality measures (gene complexity, gene detection rate, average gene expression) were performed using 40000 samples UMI counts per cell. Overall design: Single cells were sorted in 96, wells plates. Per condition (unfixed or RAID) three sets (A,B,C) of 48 cells were processed with the CELseq2 protocol.

Sample: Unfixed_SetC

SAMN09429726 • SRS3421894 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Reverse Transcription from detergent and heat based lysates, according to the SORT-seq/CELseq2 protocol CELseq2 library prep

Experiment attributes:

GEO Accession: GSM3192001

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 21.1M spots, 1.6G bases, 622.5Mb

Run	# of Spots	# of Bases	Size	Published
SRR7347828	21,087,841	1.6G	622.5Mb	2019-02-15

ID:: 5708206

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